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The quality of DNA extracted manually is higher than the quality of DNA extracted by the automatic nucleic acid extractor. There is no obvious difference in the quality of DNA obtained by different personnel operating the automatic nucleic acid extractor. The quality of DNA extracted manually by different personnel is significantly different. The reason may be manual The extracted DNA is different due to the different operating habits and proficiency of each person, which leads to larger errors, which makes the quality of the extracted DNA vary greatly. However, the automatic nucleic acid extractor has less human factors and is more objective, so each extraction The DNA quality is relatively stable, and the difference is not obvious.
However, whether it is manually extracted DNA or DNA extracted by an automatic nucleic acid extractor, the OD260/OD280 are maintained between 1.7 and 2.2, which meets the requirements for PCR testing of paraffin specimens. The advantage of the automatic nucleic acid extractor for extracting DNA is that even if different personnel operate the instrument, the quality and purity of the extracted DNA are relatively stable, and it avoids human error and labor input in the manual extraction process; the disadvantage is that the quality of the extracted DNA is better than manual extraction. The efficiency of DNA extraction is low, and the DNA content of small specimens is inherently low. The quality of DNA extracted by the automatic nucleic acid extractor is too low, which may make the specimens unusable for molecular pathology testing; the advantage of manual DNA extraction lies in the quality of the extracted DNA High, short time. For samples with low DNA content, the purification volume can be adjusted artificially, thereby improving the quality of DNA, which is more conducive to the molecular pathology detection of small clinical specimens; the disadvantage is that the quality of DNA extracted by different personnel is obviously different and the uncertainty is relatively large . Manual operation is closely related to the proficiency and care of each operator. Instrument testing is more objective, consistent, and easier to standardize.
Company Details
Bronze Gleitlager
,
Bronze Sleeve Bushings
and
Graphite Plugged Bushings
from Quality China Factory
Business Type:
Distributor/Wholesaler,Trading Company
Year Established:
2016
Total Annual:
1800000-2000000
Employee Number:
80~90
Ecer Certification：
Active Member
The company uses the business philosophy that is fully integrated with the market economy and efficient management and operation mechanism, takes the market as the guide, and builds a "customer-centric" marketing system based on customer needs. Ensure to provide customers with high-quality... The company uses the business philosophy that is fully integrated with the market economy and efficient management and operation mechanism, takes the market as the guide, and builds a "customer-centric" marketing system based on customer needs. Ensure to provide customers with high-quality...
