Payment Terms | T/T, Western Union |
Supply Ability | 100000kit/month |
Delivery Time | 20-35days |
Packaging Details | 96T/kit |
Method | Elisa |
Format | 96T/KIT |
Valid | 24 month |
Accuracy | 99.84% |
Sensitivity | 99.83% |
Application | HIV 1/2 antibody elisa |
Brand Name | New Life |
Model Number | 96 wells |
Certification | ISO, CE |
Place of Origin | China |
View Detail Information
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Product Specification
Payment Terms | T/T, Western Union | Supply Ability | 100000kit/month |
Delivery Time | 20-35days | Packaging Details | 96T/kit |
Method | Elisa | Format | 96T/KIT |
Valid | 24 month | Accuracy | 99.84% |
Sensitivity | 99.83% | Application | HIV 1/2 antibody elisa |
Brand Name | New Life | Model Number | 96 wells |
Certification | ISO, CE | Place of Origin | China |
High Light | elisa diagnostic kits ,elisa assay kit |
HIV 1/2 antibody elisa test kitt,96wells/kit, high accuracy Elisa Sandwich method, for quantitative measurement
Materials provided with the kit:
96T | ||
Coated Microtiter Plate | 1 bag | 12*8 |
Conjugate | 1vial | 10.5mL |
Biotin Working Liquid | 1vial | 3mL |
Wash Buffer Concentrate (40*) | 1vial | 20mL |
Substrate A | 1vial | 7mL |
Substrate B | 1vial | 7mL |
Stop Solution | 1vial | 6mL |
Negative Control | 1vial | 1mL |
Positive Control | 1vial | 1mL |
Closure Plate Membrane | 3sheet |
Note: different batches of reagent kit, and different component can not be exchanged for use. Once open, stable for 3 months at 2-8°C.
INTENDED USE
The Anti-HIV 1+2 ELISA is a qualitative enzyme immunoassay for the in vitro detection of Human
Immunodeficiency Virus (HIV) infection, based on the detection of antibodies to HIV-1 and HIV-2 in human serum or plasma. It is intended for screening of blood donors and diagnosing patients related to infection with HIV.
ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer with 19 volumes of distilled water,
mix well.
2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50ul of Sample Diluent , dispense 50 ul of sample or negative control or positive control to the respective wells. Gently vibrating the plate.
3. Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37 for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add 100ul of conjugate to each well (except the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37 for 30 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50ul of Substrate Solution A and 50 ul of Substrate Solution B to each well, mix well.
9. Stop reaction: Add 50ul Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
Name | HIV 1,2 ELISA Test |
Full name | Human Immunodeficiency Viruses Type 1,2 Antibody (HIV1,2) ELISA Test Kit |
Category Name | HIV ELISA kit |
Test | 96 Tests |
Method | ELISA: Enzyme Linked Immunosorbent Assay |
Principle | ELISA: Sandwich; Double antigen |
Detection Range | Qualitative: Positive, negative and cutoff calibrators |
Sample | 100 uL Serum or plasma |
Specificity | 99.89% |
Sensitivity | 99.95% |
Total Time | 15 Min |
Shelf Life | 12 Months from the manufacturing date |
ORIENT NEW LIFE MEDICAL CO., LTD. | |
Contact: | Jerry Meng |
Email: | Jerry @ newlifebiotest .com |
Tel. | +86 18657312116 |
SKYPE | enetjerry |
Company Details
Business Type:
Manufacturer,Exporter
Year Established:
2009
Total Annual:
10,000,000-15,000,000
Employee Number:
200~300
Ecer Certification:
Active Member
Established in 2009, Orient New Life Medical Co., Ltd., is specialized in high-tech biological diagnostic test development, research and production, as well as high-tech medical device development, research and production. New Life has built up two production base, which produce biological diagnosti... Established in 2009, Orient New Life Medical Co., Ltd., is specialized in high-tech biological diagnostic test development, research and production, as well as high-tech medical device development, research and production. New Life has built up two production base, which produce biological diagnosti...
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