125mL PCR Virus Nucleic Acid Extraction Kit For RT PCR Digestion

Product Specification

125mL PCR Virus Nucleic Acid Extraction Kit For RT PCR Digestion

Disposable PCR virus nucleic acid extraction kit nucleic acid purification extraction kit manufacturer

product description:

HUACHENYANG DNA Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, as well as from oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored at room temperature for up to 30 days in our proprietary storage buffer before processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/ Chloroform extraction or alcohol precipitation yields high-quality genomic DNA within 15 minutes, with an average DNA yield of 8 μg per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions

Description:

The main components are magnetic beads, proteinase K, sodium chloride. Magnesium Chloride. Potassium chloride, Tris, etc.

Features:

Using silicon magnetic beads and buffer system with unique separation function, it can quickly, highly sensitively and efficiently extract high-purity nucleic acid from samples, and the extracted and purified nucleic acid can be used for PCR, RT-PCR, digestion, reverse transcription, and Southern Various common downstream experiments such as blot, applicable specimen types: whole blood, tissue homogenate, swab, serum, plasma, bronchoalveolar lavage fluid, etc. Applicable extraction instruments: most of the automatic nucleic acid extraction instruments on the market

benefit:

1. Magnetic bead-based technology isolates genomic DNA without the need for hazardous chemicals, centrifugation or vacuum manifolds, phenol and ethanol precipitation.

2. Fast and efficient purification of genomic DNA from human buccal swabs in less than 15 minutes after sample preparation and lysis.

3. Simple cleavage with proteinase K without any mechanical cleavage.

4. Minimal RNA contamination.

5. Purified genomic DNA exhibits improved downstream performance in applications such as PCR.

6. A kit is included for automated processing of large numbers of samples in 96-well plates using a liquid handling robot.

Product Usage:

Nucleic acid extraction or purification reagents are used for nucleic acid extraction, enrichment, purification and other steps. The processed product is used for clinical in vitro detection.

Product Instructions:

The magnetic bead material specifically adsorbs and dissociates from nucleic acid under different salt concentrations and acid-base conditions, separates nucleic acid and other components in the lysed cells by the transfer magnetic bead method, and then obtains the magnetic bead-free extract by washing and elution- -- Nucleic acids.

The first step - lysis, use lysis solution to destroy the cellular structure of animal and plant cells, so that the DNA is fully released

The second step—binding, adding magnetic bead binding solution to specifically adsorb DNA, so that DNA can be fully combined with magnetic beads;

The third step------washing, using detergent, after multiple washings, fully remove other residual impurities;

The fourth step - elution, use the eluent to dissociate the magnetic beads and DNA to obtain a higher-quality DNA solution.