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Shanghai Korain Biotech Co., Ltd

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China 96 Wells 48 Wells Bovine ELISA Kits Sandwich Highly Sensitive With Oem Service
China 96 Wells 48 Wells Bovine ELISA Kits Sandwich Highly Sensitive With Oem Service

  1. China 96 Wells 48 Wells Bovine ELISA Kits Sandwich Highly Sensitive With Oem Service

96 Wells 48 Wells Bovine ELISA Kits Sandwich Highly Sensitive With Oem Service

  1. MOQ: Negotiation
  2. Price: Negotiation
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Supply Ability Western Union, T/T
Delivery Time 1-3 business days, bulk order within one week
Packaging Details Wrapped with ice pack and styrofoam package
Organism Species Bovine
Quality CE, ISO
Sample serum,plasma,urine,tissue,cell culture supernatant
Known as TSH
Shipping DHL/FedEX
Assay Principle Sandwich
Brand Name BT Lab
Model Number Cat.No E0217Bo
Certification CE, ISO9001:2015, MSDS
Place of Origin Shanghai, China

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Product Specification

Supply Ability Western Union, T/T Delivery Time 1-3 business days, bulk order within one week
Packaging Details Wrapped with ice pack and styrofoam package Organism Species Bovine
Quality CE, ISO Sample serum,plasma,urine,tissue,cell culture supernatant
Known as TSH Shipping DHL/FedEX
Assay Principle Sandwich Brand Name BT Lab
Model Number Cat.No E0217Bo Certification CE, ISO9001:2015, MSDS
Place of Origin Shanghai, China
High Light sandwich elisa kitelisa test kit

96 Wells 48 Wells Bovine TSH ELISA Assay Kit Sandwich Highly Sensitive With Oem Service
 

Cat.No E0217Bo

Standard Curve Range: 0.05mIU/L - 30mIU/L

Sensitivity: 0.027mIU/L

Size: 96 wells

Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

*This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

 

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

CV(%) = SD/mean x 100

Intra-Assay: CV<8%

Inter-Assay: CV<10%

 

Assay Principle

This Sandwich ELISA Kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine TSH antibody. TSH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine TSH Antibody is added and binds to TSH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TSH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine TSH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

 

Reagent Provided

Components Quantity
Standard Solution (32mIU/L) 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (30x) 20ml x1
Biotinylated Bovine TSH Antibody 1ml x1
User Instruction 1
Plate Sealer 2 pics
Zipper bag 1 pic

 

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (32mIU/L) with 120μl of standard diluent to generate a 16mIU/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (16mIU/L) 1:2 with standard diluent to produce 8mIU/L, 4mIU/L, 2mIU/L and 1mIU/L solutions. Standard diluent serves as the zero standard(0 mIU/L). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

 

16mIU/L Standard No.5 120μl Original Standard + 120μl Standard Diluent
8mIU/L Standard No.4 120μl Standard No.5 + 120μl Standard Diluent
4mIU/L Standard No.3 120μl Standard No.4 + 120μl Standard Diluent
2mIU/L Standard No.2 120μl Standard No.3 + 120μl Standard Diluent
1mIU/L Standard No.1 120μl Standard No.2 + 120μl Standard Diluent

 

Standard Concentration Standard No.5 Standard No.4 Standard No.3 Standard No.2 Standard No.1
32mIU/L 16mIU/L 8mIU/L 4mIU/L 2mIU/L 1mIU/L

 

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 600 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

 

Assay Procedure

1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

4. Add 40μl sample to sample wells and then add 10μl anti-TSH antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells (Not blank control well). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.

 

Summary

1. Prepare all reagents, samples and standards.

2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

3. Wash the plate 5 times.

4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

5. Add stop solution and color develops.

6. Read the OD value within 10 minutes.

Company Details

Bronze Gleitlager

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 from Quality China Factory
  • Business Type:

    Manufacturer,Distributor/Wholesaler,Exporter,Seller

  • Year Established:

    2010

  • Ecer Certification:

    Active Member

Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researc... Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researc...

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Get in touch with us

  • Reach Us
  • Shanghai Korain Biotech Co., Ltd
  • #1008.Junjiang Internatioanl Bldg. 228 Ningguo Rd. Yangpu Dist. Shanghai. 200090. China
  • https://www.elisatest-kit.com/

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