Shanghai Korain Biotech Co., Ltd
| Payment Terms | Western Union, T/T |
| Supply Ability | In Stock |
| Delivery Time | 1-3 business days, bulk order within one week |
| Packaging Details | Wrapped with ice pack and styrofoam package |
| Target Protein | Osteopontin |
| Sample | serum,plasma,urine,tissue,cell culture supernatant |
| Assay Time | 2 hours |
| Shipping | DHL/FedEX |
| Lead Time | Within 48 hours |
| Assay Principle | Sandwich |
| Brand Name | BT Lab |
| Model Number | Cat.No E0193Rb |
| Certification | CE, ISO9001:2005, MSDS |
| Place of Origin | Shanghai, China |
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Product Specification
| Payment Terms | Western Union, T/T | Supply Ability | In Stock |
| Delivery Time | 1-3 business days, bulk order within one week | Packaging Details | Wrapped with ice pack and styrofoam package |
| Target Protein | Osteopontin | Sample | serum,plasma,urine,tissue,cell culture supernatant |
| Assay Time | 2 hours | Shipping | DHL/FedEX |
| Lead Time | Within 48 hours | Assay Principle | Sandwich |
| Brand Name | BT Lab | Model Number | Cat.No E0193Rb |
| Certification | CE, ISO9001:2005, MSDS | Place of Origin | Shanghai, China |
| High Light | elisa sandwich assay kit ，sandwich assay | ||
Rabbit Osteopontin ELISA Kit with High Spesificity and Sensitivity
Cat.No E0193Rb
Standard Curve Range: 0.5ng/ml - 150ng/ml
Sensitivity: 0.26ng/ml
Size: 96 wells
Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
* This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.
Intended Use
This sandwich kit is for the accurate quantitative detection of Rabbit Osteopontin (also known as OPN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Assay Principle
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit OPN antibody. OPN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit OPN Antibody is added and binds to OPN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OPN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit OPN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Reagent Provided
| Components | Quantity |
| Standard Solution (160ng/ml) | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (30x) | 20ml x1 |
| Biotinylated Rabbit OPN Antibody | 1ml x1 |
| User Instruction | 1 |
| Plate Sealer | 2 pics |
| Zipper bag | 1 pic |
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (160ng/ml) with 120μl of standard diluent to generate a 80ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (80ng/ml) 1:2 with standard diluent to produce 40ng/ml, 20ng/ml, 10ng/ml and 5ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:
Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.
Company Details
Business Type:
Manufacturer,Distributor/Wholesaler,Exporter,Seller
Year Established:
2010
Ecer Certification：
Active Member
Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researc... Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researc...
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