Ce Approved Rapid Test Kit / Virus Test Kit For Extraction Of Nucleic Acid

(Please read the instructions carefully before use)

[ Product name ]

Virus Nucleic Acid Purification Reagents

[Product types] 50 tests/ kit, 100 tests/ kit, 200 tests/ kit

[Item NO.] T210

[Expected usage]

For extraction, enrichment and purification of nucleic acid. The processed product is used for clinical in vitro detection.

[Product introduction]

Lysis Buffer breaks up the virus and release Denaturation. The virus DNA/RNA can be adsorbed and eluted through polymer membrane material .

[Composition]

※1: For 50 tests/kit, mix 12 mL anhydrous ethanol and 18 mL deproteinized rinse, and mix 48 mL anhydrous ethanol and 12 mL scrubbing solution before use.

For 100 tests/kit, mix 24 mL anhydrous ethanol and 36 mL deproteinized rinse, and mix 96 mL anhydrous ethanol and 24 mL scrubbing solution before use.

For 200 tests/kit,mix 48 mL anhydrous ethanol and 72 mL deproteinized rinse , and mix 192 mL anhydrous ethanol and 48 mL scrubbing solution before use.

2: Others: Anhydrous Ethanol (Analytical Purity)

[ Storage and Expiration date]

2~8 ℃ stable. Transport at room temperature for 14 days at most. It has a shelf life of 12 months.

[ Applicable instruments ]

Centrifuge (maximum speed ≥12,000g), thermostatic metal bath, vortex oscillator.

[ Sample requirement ]

Virus DNA / RNA was extracted from whole blood, serum, plasma, diseased materials, feces and body fluid.

If the volume of liquid sample less than 200 μ L, add PBS buffer or saline to 200μL. [Steps]

A. Sample preparation

1. Directly sampled for whole blood, plasma, serum, ascites, oral fluid and other liquid samples.

2. For the virus in animal and plant tissues: add saline or PBS, grind thoroughly, centrifuge 12000g for 5~10min, remove and set aside.

3. For the virus in fecal samples: add saline or PBS, mix thoroughly, 12000g centrifuged for 5-10min, remove and set aside.

B. Reagent preparation

Room temperature stable. Keep the cracking solution at 56 ℃ in a water bath for 10 minutes while crystallization in the low temperature and ensure that the salt released fully dissolved.

C. Sample Extraction Operations

1. Add 10 μL protease K to 1.5 mL centrifuge tube,add 200μL processed sample to the centrifuge tube (if less than 200μL, please use PBS buffer or saline to make up to

200μL), then add 200 μ L lysate. Cover the tube and oscillate for 30 seconds.

2. Incubation at 56 ℃ for 15 minutes.

3. Add 250 μL anhydrous ethanol to the centrifuge tube, cover the tube, and oscillate for 15 seconds. Centrifugate and collect the liquid from the wall.

4. Transfer the above mixture to the purification column, centrifuge at 10,000 g for 1 minute, and discard the liquid in the receiving tube.

5. Add 500 μL deproteinized rinse solution to the purification column, centrifuge at 10,000 g for one minute, and discard the liquid in the receiving tube.

6. Add 500 μL scrubbing solution to the purification column, centrifuge at 10,000 g for 1 minute and discard the liquid in the receiving tube. Kill.

2.Add 500μL scrubbing solution to the purification column, centrifuge at 10,000 g for 1 minute and discard the liquid in the receiving tube.

2. Put the purification column back into the liquid receiving pipe, centrifugate 10,000 g again for 2 minutes.

3. Transfer the column to a new 1.5 mL centrifuge tube, add 50~100 μL eluent to the column, and incubate at room temperature for 2 minutes.

4. After 12,000 G centrifugation for a minute and discarding the purification column, 1.5 mL of the liquid in the centrifuge tube contained DNA / RNA. The extracted DNA / RNA can be used for various downstream applications. If not, please store it at-80 ℃. [Interpretation of test results]

Suitable for samples of whole blood, serum, plasma, disease material, stool and body fluids.

[Limitations of the inspection method ]

Volume of liquid sample: Less than 200 μL;

Requires high-sensitivity PCR detection reagents

[Product performance indicators]

The sensitivity was 10 IU / mL by a high-sensitivity HBV DNA detection reagent and the linear range was 10 IU / mL-10 IU /mL. The sensitivity was 100 IU / mL by a high-sensitivity HCV RNA detection reagent and the linear range was 10 IU / mL- 10 IU /mL. The quality control products that have been calibrated by national standards are repeatedly determined.

[ Notes ]

1. Please check the crystallization before use. If so,keep the cracking solution at 56 ℃ and shaken constantly until the crystallization dissolve completely.

2. Add absolute ethanol to the protein-free rinsing solution and washing solution as indicated.