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The ASFV P30/P54/P72 Antibody Combo Rapid Test Cassette (Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of P30 antibody, P54 antibody and P72 antibody to African swine fever virus in serum or plasma.
P30 protein, P54 protein and P72 protein are expressed in the early, middle and late stage of ASFV infection. The detection of corresponding antibodies can monitor the complete infection cycle and evaluate the infection process.
PRINCIPLE
The ASFV P30/P54/P72 Antibody Combo Rapid Test Cassette (Serum/Plasma) is a rapid chromatographic immunoassay based on double antigen sandwich format for the qualitative detection of P30 antibody, P54 antibody and P72 antibody to African Swine Fever Virus in serum or plasma. The test cassette has three testing windows. Each testing window has T (test) region and C (control) region.
The membrane is pre-coated with ASFV antigens. During testing, antibody to ASFV, if present in serum or plasma specimen, will react with ASFV antigen coated colloidal gold particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with ASFV antigens on the membrane in the test line region. If the specimen contains antibody to African Swine Fever Virus, a colored line will appear in the test line region indicating a positive result.
If the specimen does not contain African Swine Fever Virus antibody, a colored line will not appear in the test line region indicating a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
DIRECTIONS FOR USE
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed
within one hour.
2. Separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below - 20 °C.
3. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 μl) to the specimen well (S) of the test cassette, then quickly add 2 drops of buffer and start the timer. See illustration below.
4. Read the results at 10 minutes. Do not interpret results after 20 minutes.
INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line (any testing window), regardless of T line being strong or faint.
Negative: Only clear C line appears.
Invalid: No colored line appears in C region, regardless of T line’s appearance.
Order Information:
Cat. No.
Product
Specimen
Pack
VIASF-335
ASFV P30/P54/P72 Antibody Combo Rapid Test
Serum/Plasma
10T
Company Details
Bronze Gleitlager
,
Bronze Sleeve Bushings
and
Graphite Plugged Bushings
from Quality China Factory
Business Type:
Manufacturer,Exporter,Trading Company
Total Annual:
80000000-100000000
Employee Number:
500~10000
Ecer Certification：
Verified Supplier
CITEST DIAGNOSTICS INC., an emerging In Vitro Diagnostics company based in Canada, is experiencing growth on a global scale, engaging in multinational operations. CITEST was founded by highly experienced IVD industry professionals with proven track records building and growing successful companies.
... CITEST DIAGNOSTICS INC., an emerging In Vitro Diagnostics company based in Canada, is experiencing growth on a global scale, engaging in multinational operations. CITEST was founded by highly experienced IVD industry professionals with proven track records building and growing successful companies.
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