Biovantion Inc.
| Payment Terms | L/C, Western Union, T/T |
| Supply Ability | 50000 Box/Month |
| Delivery Time | 5-8 working days |
| Packaging Details | Carton |
| Specimen | Serum or Plasma |
| Read Time | 60 Minutes |
| Storage | 2-8℃ |
| EXP | 18 Months |
| Size | 96 Test/Kit |
| Brand Name | BIOVANTION |
| Model Number | 96 Test/Kit |
| Certification | ISO13485 |
| Place of Origin | CHINA |
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Product Specification
| Payment Terms | L/C, Western Union, T/T | Supply Ability | 50000 Box/Month |
| Delivery Time | 5-8 working days | Packaging Details | Carton |
| Specimen | Serum or Plasma | Read Time | 60 Minutes |
| Storage | 2-8℃ | EXP | 18 Months |
| Size | 96 Test/Kit | Brand Name | BIOVANTION |
| Model Number | 96 Test/Kit | Certification | ISO13485 |
| Place of Origin | CHINA | ||
| High Light | 60 Minutes HIV Ab Ag Test ，Hiv 4th Generation Assay ELISA Kit ，ISO13485 HIV Ag Ab Test | ||
Hot Sale HIV Ab & Ag ELISA 96 Test/Kit
Catalog No.: BE701A
1. INTENDED USE
2. Materials provided with the kits:
| Items | Description | 96T |
| 1. | Microplate | 96 wells |
| 2. | Negative Control | 1 mL ×1 |
| 3. | Positive Control HIV-1 | 1 mL ×1 |
| 4. | Positive Control HIV-2 | 1 mL ×1 |
| 5. | Positive Control P24 Antigen | 1 mL ×1 |
| 6. | Conjugate 1 | 3 mL ×1 |
| 7. | Conjugate 2 | 12 mL ×1 |
| 8. | Washing Buffer | 40 mL ×1 |
| 9. | Substrate Solution A | 6 mL ×1 |
| 10. | Substrate Solution B | 6 mL ×1 |
| 11. | Stop Solution | 6 mL ×1 |
| 12. | Plate Cover | 3 piececs |
| 13. | Insert | 1 copy |
3. ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Washing Buffer with 19 volumes of distilled water, mix well.
2. Add Conjugate 1 and Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2
wells as Negative Control, 2 wells as Positive Control HIV-1, 2 wells Positive Control HIV-2 and 2 wells
Positive Control P24 Antigen. After dispensing 75μL of sample or Negative Control or Positive Control to
the respective wells, dispense 25μL of Conjugate 1 to each well (except the blank well). Gently vibrating
the plate.
3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled
water-bath or microplate incubator at 37℃ for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer (10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate 2: Add 100μL of Conjugate 2 to each well (except the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well.
Cover and incubate at 37℃ for 30 minutes.
9. Stop reaction: Add 50μL Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.
Company Details
Business Type:
Manufacturer,Importer,Exporter,Trading Company,Seller
Ecer Certification：
Verified Supplier
Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir... Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir...
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