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{"title":"Human Anti HIV 1&2 Elisa Test Kit For Human Immunodeficiency Virus","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/90\/77\/469f316ca3cf2d34572584052249-200x200-1\/human_anti_hiv_1_26amp_3b2_elisa_test_kit_for_human_immunodeficiency_virus.jpg","attrs":{"Brand Name":"BIOVANTION","Model Number":"BE601A","Certification":"ISO13485","Place of Origin":"CHINA"}}
{"title":"BIOVANTION Anti Thyroglobulin Antibody Test Anti Tg Ab Test Kit","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/9b\/e9\/34134fbf10ec4d4fa07bf5173468-200x200-1\/biovantion_anti_thyroglobulin_antibody_test_anti_tg_ab_test_kit.jpg","attrs":{"Brand Name":"BIOVANTION","Model Number":"BD-06E","Certification":"ISO13485","Place of Origin":"CHINA"}}
{"title":"Anti-TPO ELISA TEST KIT(Anti-Thyroid Peroxidase)","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/a3\/a7\/4f404e1e299ef5cf80a2bd43dae3-200x200-1\/anti_tpo_elisa_test_kit_28anti_thyroid_peroxidase_29.jpg","attrs":{"Brand Name":"BIOVANTION","Model Number":"96 Test\/Kit","Certification":"ISO13485","Place of Origin":"CHINA"}}
{"title":"HAV IgG Elisa Kit Antibody Diagnostic Kit For Hepatitis A Virus","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/2b\/b7\/db8f15e9f0da8259146d24f2a5a7-200x200-1\/hav_igg_elisa_kit_antibody_diagnostic_kit_for_hepatitis_a_virus.jpg","attrs":{"Brand Name":"BIOVANTION","Model Number":"BE302A","Certification":"ISO13485","Place of Origin":"CHINA"}}
{"title":"HEV Human Igg Elisa Kit Diagnostic For IgG Antibody To Hepatitis E Virus","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/9f\/1f\/493b886d1280f15337f6469fe5be-200x200-1\/hev_human_igg_elisa_kit_diagnostic_for_igg_antibody_to_hepatitis_e_virus.jpg","attrs":{"Brand Name":"BIOVANTION","Model Number":"BE401A","Certification":"ISO13485","Place of Origin":"CHINA"}}
{"title":"HEV IgM Elisa Test Kit Serum Vs Plasma For Antibody Detection","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/66\/1f\/8c9a0a90951c5b37c8e02845e2a0-200x200-1\/hev_igm_elisa_test_kit_serum_vs_plasma_for_antibody_detection.jpg","attrs":{"Brand Name":"BIOVANTION","Model Number":"BE402A","Certification":"ISO13485","Place of Origin":"CHINA"}}
{"title":"EBV-VCA IgA Ab Test ELISA Kit Enzyme Immunoassay Test Plasma Specimen","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/53\/0f\/df225ab351d64813f86d33fd68ec-200x200-1\/ebv_vca_iga_ab_test_elisa_kit_enzyme_immunoassay_test_plasma_specimen.jpg","attrs":{"Brand Name":"BIOVANTION","Model Number":"BE501A","Certification":"ISO13485","Place of Origin":"CHINA"}}
Human High Quality Anti-HIV 1&2 ELISA Kit 96Test/Kit The third generation double antigen sandwich method to detect antibody to HIV1+2 in serum or plasma 1.SUMMARY AND PRINCIPLE OF THE TEST
Human Immunodeficiency Virus Type (HIV-1) has been isolated from patients with AIDS and AIDS-related complex (ARC). HIV-1 was thought to be the sole causive agent of these syndromes until 1986, when a second type of Human Immunodeficiency Virus (HIV-2) was isolated and also reported to cause AIDS. Since the initial discovery, more than 600 cases of HIV-2 infection have been documented worldwide, with over 40 cases of AIDS related to HIV-2.
Both viruses have the same morphology and lymphotropism, and the modes of transmission appear to be identical. In addition, HIV-1 and HIV-2 genomes exhibit about 60% homology in conserved genes such as gag and pol. Serologic studies have also shown that the core proteins of HIV-1 and HIV-2 display frequent cross-reactivity whereas the envelope proteins are more type-specific.
2. Materials provided with the kits:
Item
Description
96T
480T
1
Microtiter Well
1
5
2
Sample Diluent
6ml
30ml
3
Enzyme Conjugate
12ml
60ml
4
Negative Control
1ml
5ml
5
Positive Control(HIV-1)
1ml
5ml
6
Positive Control(HIV-2)
1ml
5ml
7
Wash Buffer Concentrate (20x)
30ml
150ml
8
Substrate Solution A
6ml
30ml
9
Substrate Solution B
6ml
30ml
10
Stop Solution
6ml
30ml
11
User manual
1 copy
5 copy
3. ASSAY PROCEDURE
Allow all components to reach room temperature before use. Dilute concentrated wash buffer 1:20 with ddH2O.
Set two positive and three negative controls. Dispense 100μl of Positive Control(HIV-1 and HIV-2) as well as Negative Control in duplicate into respective wells without sample diluent. Set one blank well as background control without adding any liquid. Add 50μl of sample diluent into each test well, add 50 μl test serum or plasma into test wells mix thoroughly.
Place the microtiter plate into a humidified box and incubate at 37°C for 30 min.
Discard reaction solution and add 350μl diluted wash buffer to each well. Discard the wash buffer after soaking for 15-30seconds. Repeat the washing procedure 5 times and then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for a few seconds.
Add 100μl of Enzyme Conjugate to each well. Mix it gently by swirling the microtiter plate on flat bench for 1 min. Do not add Enzyme Conjugate to the blank well..
Place the microtiter plate into a humidified box and incubate at 37°C for 30 min.
Wash each well 5 times by filling each well with diluted 1X wash buffer, then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for a few seconds.
Add 50μl of Substrate Solution A (HRP substrate) to each well, then add 50μl of Substrate Solution B (TMB) to each well. Mix gently and incubate at 37°C for15 min.
Add one drop (50μl) of Stop Solution to each well to stop the color reaction.
Read the OD value at 450 nm/630 nm with dual filter plate reader. It is option to read the OD value at 450 nm with single filter plate reader. (using the OD value of the blank well to correct all the OD reading from all wells
Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir... Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir...
