Payment Terms | L/C, T/T, Western Union |
Supply Ability | 100,000 per day |
Delivery Time | depending on order quantity |
Packaging Details | Carton Package |
Specimen | Feline/dog droppings. |
Packing | 8T/box |
Linear | | r | >0.980 |
LOD | 10 copies/μL |
CV | ≤5% |
Applicable | Micgene 242/244/244 IVD |
Brand Name | Biokey |
Model Number | Bik-Q-P039 |
Certification | ISO13485 |
Place of Origin | Guangzhou China |
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Product Specification
Payment Terms | L/C, T/T, Western Union | Supply Ability | 100,000 per day |
Delivery Time | depending on order quantity | Packaging Details | Carton Package |
Specimen | Feline/dog droppings. | Packing | 8T/box |
Linear | | r | >0.980 | LOD | 10 copies/μL |
CV | ≤5% | Applicable | Micgene 242/244/244 IVD |
Brand Name | Biokey | Model Number | Bik-Q-P039 |
Certification | ISO13485 | Place of Origin | Guangzhou China |
High Light | Dog Helicobacter Test Kit ,Helicobacter Test Kit PCR ,Canine h pylori DNA Detection Kit |
Canine / Dog Helicobacter pylori ( Hp ) Bacteria DNA Detection Kit ( PCR-Fluorescent Probe )
Intend for Use:
This kit is suitable for the specific detection of pathogenic Helicobacter pylori (Hp) in cat/dog feces samples.
Principle:
This kit selection of helicobacter pylori (Hp) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to achieve the detection of Helicobacter pylori nucleic acid (Hp) in the completely closed reaction system.
Component:
Composition | Quantity |
PCR Liquid | 8 tube |
Positive control | 1 tube |
Negative control | 1 tube |
Sample requirements:
1. Sample type
Cat/dog droppings.
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 94℃ | 3min |
2 | 45 | 94℃ | 10s |
60℃ | 20s collection fluorescent |
FAM was selected for detection channel
Positive judgment value or reference interval:
Results | Ct Value |
Positive | Ct≤38 |
Negative | No Ct value or Ct value≥45 |
Gray area | 38<Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | >0.980.
Product picture:
Company Details
Business Type:
Manufacturer,Distributor/Wholesaler,Exporter,Seller
Year Established:
2003
Total Annual:
5M-10M
Employee Number:
100~200
Ecer Certification:
Verified Supplier
Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n... Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n...
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