Guangzhou BioKey Healthy Technology Co.Ltd
| Payment Terms | L/C, T/T, Western Union |
| Supply Ability | 100,000 per day |
| Delivery Time | Depending on order quantity |
| Packaging Details | Carton package |
| Package | 24T/Box |
| Validity | 12 months |
| Storage | at -20±5℃, protected from light |
| Applicable | Micgene 242 / 244 、ABI QuantStudio5 |
| Mini limit | 10 copies/μL |
| Linear | | r | > 0.980 |
| Brand Name | Biokey |
| Model Number | BIK-Q-W011 |
| Certification | ISO 13485 |
| Place of Origin | Guangzhou China |
View Detail Information
Explore similar products
Molecular Detection Of Aquatic Pathogens Fluorescent Quantitative PCR Diagnostic
Infectious Myonecrosis Virus Dna Amplification Kit IMNV Nucleic Acid Detection
Aquaculture Bacterial Vibrio detection Medical Laboratory Real Time PCR
Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman
Product Specification
| Payment Terms | L/C, T/T, Western Union | Supply Ability | 100,000 per day |
| Delivery Time | Depending on order quantity | Packaging Details | Carton package |
| Package | 24T/Box | Validity | 12 months |
| Storage | at -20±5℃, protected from light | Applicable | Micgene 242 / 244 、ABI QuantStudio5 |
| Mini limit | 10 copies/μL | Linear | | r | > 0.980 |
| Brand Name | Biokey | Model Number | BIK-Q-W011 |
| Certification | ISO 13485 | Place of Origin | Guangzhou China |
| High Light | YHV Taqman Quantitative PCR Kit ，Taqman Yellow Head Virus Rna Test Kit ，YHV Rna Test Kit | ||
Yellow Head Virus (YHV) Nucleic Acid Detection Kit (RT-PCR-Fluorescent Probe Method)
Intended use:
This kit is suitable for the specific detection of pathogenic yellow head virus (YHV) in tissue samples such as gills, antennae glands, hematopoietic tissues and lymphatic organs of penaeus prawn.
Principle:
This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat goblet virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of Prawn yellow head virus (YHV) was detected by fluorescence PCR in a completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 24 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
1. Sample type
Tissue samples from prawn
2. Sample collection
Samples were collected according to SNT 1151.4-2003.
Take fresh prawn tissue as sample.
2.1 For larvae or young shrimp, whole shrimp or shrimp heads are taken as samples. To remove the head, use sterilizing scissors and tweezers to remove the eyes, breastplate, all thoracic appendages, and abdomen behind the head.
2.2 For adult shrimp, gill filaments, lymphatic organs or hemolymph were taken as samples.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 50℃ | 20min |
| 2 | 1 | 95℃ | 2min30s |
| 3 | 45 | 95℃ | 10s |
| 60℃ | 30s collect the fluorescent |
FAM is selected for detection channels.
Quality control:
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 38＜Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
Operation details please check from IFU!
Company Details
Business Type:
Manufacturer,Distributor/Wholesaler,Exporter,Seller
Year Established:
2003
Total Annual:
5M-10M
Employee Number:
100~200
Ecer Certification：
Verified Supplier
Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n... Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n...
Get in touch with us
Leave a Message, we will call you back quickly!