Guangzhou BioKey Healthy Technology Co.Ltd
| Payment Terms | L/C, T/T, Western Union |
| Supply Ability | 100,000 per day |
| Delivery Time | Depending on order quantity |
| Packaging Details | Carton package |
| Package | 24T/Box |
| Validity | 18 months |
| Specimen | Fish fry or fish heart, intestine, spleen, kidney and other visceral tissue tissue samples. |
| Applicable | Micgene 242 / 244 、ABI QuantStudio5 |
| Mini limit | 10 copies/μL |
| Positive | Ct≤38 |
| Brand Name | Biokey |
| Model Number | BIK-Q-W025 |
| Certification | ISO 13485 |
| Place of Origin | Guangzhou China |
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Product Specification
| Payment Terms | L/C, T/T, Western Union | Supply Ability | 100,000 per day |
| Delivery Time | Depending on order quantity | Packaging Details | Carton package |
| Package | 24T/Box | Validity | 18 months |
| Specimen | Fish fry or fish heart, intestine, spleen, kidney and other visceral tissue tissue samples. | Applicable | Micgene 242 / 244 、ABI QuantStudio5 |
| Mini limit | 10 copies/μL | Positive | Ct≤38 |
| Brand Name | Biokey | Model Number | BIK-Q-W025 |
| Certification | ISO 13485 | Place of Origin | Guangzhou China |
| High Light | Streptococcus Test Kit Ct38 ，Streptococcus Test Kit Fluorescent Probe ，Fluorescent Probe PCR Aquaculture Kit | ||
Streptococcus iniae septicaemia virus Nucleic Acid Detection fluorescent probe PCR kit
Intended use:
This kit is suitable for the specific detection of pathogenic Streptococcus iniae septicaemia virus in fish fry or viscera tissue samples such as heart, intestine, spleen and kidney.
Principle:
This kit designed primers with Streptococcus iniae septicaemia virus specific fragment to probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Streptococcus iniae septicaemia virus in the totally closed reaction system was realized by fluorescence PCR instrument.
Main components:
| Component | Specification |
| PCR reaction liquid | 24 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
1. Sample type
Fish fry or fish heart, intestine, spleen, kidney and other visceral tissue tissue samples.
2. Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 94℃ | 3min |
| 2 | 45 | 94℃ | 10s |
| 60℃ | 20s collect fluorescent |
FAM was selected as the detection channel.
Quality control:
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 38＜Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
Product picture:
Operation details please check from IFU!
Company Details
Business Type:
Manufacturer,Distributor/Wholesaler,Exporter,Seller
Year Established:
2003
Total Annual:
5M-10M
Employee Number:
100~200
Ecer Certification：
Verified Supplier
Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n... Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n...
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