Payment Terms | L/C, T/T, Western Union |
Supply Ability | 100,000 per day |
Delivery Time | Depending on order quantity |
Packaging Details | Carton package |
Package | 8T/Box |
shelf life | 12 months |
Storage | -20±5℃ |
Applicable | Micgene 242/244/244 PCR |
LoD | 3 copies / μL |
CV | ≤5% |
Brand Name | Biokey |
Model Number | BIK-Q-P026 |
Certification | ISO 13485 |
Place of Origin | Guangzhou China |
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Product Specification
Payment Terms | L/C, T/T, Western Union | Supply Ability | 100,000 per day |
Delivery Time | Depending on order quantity | Packaging Details | Carton package |
Package | 8T/Box | shelf life | 12 months |
Storage | -20±5℃ | Applicable | Micgene 242/244/244 PCR |
LoD | 3 copies / μL | CV | ≤5% |
Brand Name | Biokey | Model Number | BIK-Q-P026 |
Certification | ISO 13485 | Place of Origin | Guangzhou China |
High Light | Canine Babesiosis Pcr Kit ,Babesiosis Pcr Kit EDTA ,Babesiosis Nucleic Acid Testing Kit |
Canine Babesiosis (B.C) Nucleic acid testing kit
(PCR-Fluorescent Probe Method)
Intended use:
This kit is suitable for the specific detection of Babesiosis (B.C) in canine EDTA whole blood and other samples.
Principle:
This kit uses the conserved gene fragment of Canine Babesiosis (B.C) to design a specific probe. The probe can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of the Taq enzyme will 5 The '-end fluorescent group is cleaved from the probe, making it free in the reaction system, thereby detaching from the 3'-end quenching group and emitting fluorescence, which is detected by a fluorescence PCR instrument to realize the detection of Canine Babesiosis (B.C) in a fully closed reaction system. Detection of Beth worm nucleic acid.
Main components:
Component | Specification |
PCR reaction liquid | 8 tubes |
Positive control | 1 tube |
Negative control | 1 tube |
Sample requirements:
1. Sample type
Canine EDTA whole blood etc.
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
Method calibration:
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 95℃ | 3min |
2 | 45 | 95℃ | 10s |
60℃ | 30s collect fluorescent |
FAM is selected for detection channels.
Positive judgment value or reference interval
Results | Ct Value |
Positive | Ct≤40 |
Negative | No Ct value or Ct value≥45 |
Gray area | 40<Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Operation details please check from IFU!
Company Details
Business Type:
Manufacturer,Distributor/Wholesaler,Exporter,Seller
Year Established:
2003
Total Annual:
5M-10M
Employee Number:
100~200
Ecer Certification:
Verified Supplier
Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n... Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team. &n...
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