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{"title":"10 - 30 \u03bcm 20% Volume Ratio 1000 mL NHS Activated Magnetic Beads","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/f5\/4e\/9541563f302f4dca75c897073e17-200x200-1\/10_30_ce_bcm_20_25_volume_ratio_1000_ml_nhs_activated_magnetic_beads.jpg","attrs":{"Brand Name":"BeaverBeads","Model Number":"70705-1000","Certification":"COA","Place of Origin":"China"}}
{"title":"Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/bb\/99\/2ef415a79bf344e91dfe93efb9cd-200x200-1\/agarose_nhs_magnetic_beads_preactivated_n_hydroxy_succinimide_nanoparticle.jpg","attrs":{"Brand Name":"BeaverBeads","Model Number":"70705-50","Certification":"COA","Place of Origin":"China"}}
{"title":"1000 mL NHS Activated Magnetic Beads 30 - 150 \u03bcm, 20% Volume Ratio","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/bb\/bd\/2220f578dce21029c0b1ac8ac14a-200x200-1\/1000_ml_nhs_activated_magnetic_beads_30_150_ce_bcm_2c_20_25_volume_ratio.jpg","attrs":{"Brand Name":"BeaverBeads","Model Number":"70702-1000","Certification":"COA","Place of Origin":"China"}}
{"title":"Agarose NHS Activated Magnetic Beads Preactivated N - Hydroxy Succinimide","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/de\/1d\/dcfdd07b8fc15bc33fa92df0eac6-200x200-1\/agarose_nhs_activated_magnetic_beads_preactivated_n_hydroxy_succinimide_nanoparticle.jpg","attrs":{"Brand Name":"BeaverBeads","Model Number":"70702-50","Certification":"COA","Place of Origin":"China"}}
{"title":"Agarose NHS Activated Magnetic Beads 20% Volume Ratio 5 mL","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/8e\/63\/2db52fb92f7405e322b759a23a6e-200x200-1\/agarose_nhs_activated_magnetic_beads_20_25_volume_ratio_5_ml.jpg","attrs":{"Brand Name":"BeaverBeads","Model Number":"70702-5","Certification":"COA","Place of Origin":"China"}}
{"title":"N - Hydroxy Succinimide Nanoparticle NHS Activated Magnetic Beads 30 - 150 \u03bcm 1","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/1b\/8b\/857bb714f88f7f7466788988f07a-200x200-1\/n_hydroxy_succinimide_nanoparticle_nhs_activated_magnetic_beads_30_150_ce_bcm_1_ml.jpg","attrs":{"Brand Name":"BeaverBeads","Model Number":"70702-1","Certification":"COA","Place of Origin":"China"}}
{"title":"1 mL Agarose NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle","imgUrl":"https:\/\/img.silkroadsl.com\/nimg\/2a\/71\/d1b8d7996a19168d24a14bf8537f-200x200-1\/1_ml_agarose_nhs_magbeads_preactivated_n_hydroxy_succinimide_nanoparticle.jpg","attrs":{"Brand Name":"BeaverBeads","Model Number":"70705-1","Certification":"COA","Place of Origin":"China"}}
The NHS magnetic beads are superparamagnetic beads with activated groups of N_Hydroxysuccinimide on the surface, which can react with primary amines on desired ligands and bind them covalently with amide linkage to the beads. The ligand can be various molecules including antigen, antibody and other proteins. Compared with the traditional carboxyl, amino magnetic beads, the NHS group of magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde, simply dissolving the amino-containing bio-ligand in the coupling buffer, at room temperature, mixing with proteins and the NHS magnetic beads for only 1-2 hours, the bio-ligand can be coupled to the magnetic beads. The advantages include easy operation, mild coupling conditions and coupling fast and efficient. The magnetic bead coupling process must be carried out in a buffer solvent free of any amino. This method can be operated manually or automatically.
2, Introduction:
Product Name
Agarose NHS Magbeads Preactivated
Mean particle size
10-30 μm
Magnetic core
Fe3O4
Shell
Agarose
Magnetic
superparamagnetism
Ligand content
~50 μM/mL gel
Binding ability
20~30 mg rabbit IgG/mL beads
Preservation fluid
20% (v/v) ethanol solution
Operation
Automate/manual
* water average particle size, Malvern Nano determination
3, FEATURES:
1. Simple, without activation, can be directly covalently coupled with the biological ligand;
2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;
3. Fast, 1 ~ 2h to complete the bio-ligand coupling;
4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;
5. Stable, the formation of a stable amide bond, to prevent the ligand off.
6. Good biocompatibility, reduced nonspecific adsorption.
4, Precautions:
1. Magnetic beads are sensitive to water. In order to ensure product quality, after sampling immediately cover
the bottle caps and seal with a sealing tape, store at 4 ℃.
2. Protein stabilizers (such as BSA, gelatin) inhibit the binding of antibodies to magnetic beads. Therefore,
during the bead-coupled antibody process, it is necessary to ensure that no primary amino-containing
protein stabilizer is present in the antibody storage system.
3. The presence of a primary amine-containing substance in the buffer inhibits protein coupled with the beads.
Removal of primary amine materials can use dialysis and desalination methods.
Company Details
Bronze Gleitlager
,
Bronze Sleeve Bushings
and
Graphite Plugged Bushings
from Quality China Factory
Business Type:
Manufacturer,Exporter,Seller
Year Established:
2011
Total Annual:
23000000-25000000
Employee Number:
80~150
Ecer Certification:
Verified Supplier
BEAVER was founded in the end of 2011, located at Suzhou Industrial Park. BEAVER combines biotech with nanotech as a “twin-turbine”strategy in the product design. BEAVER team focuses on magnetic nanoparticle technology and product development, provides customers with professional sample ... BEAVER was founded in the end of 2011, located at Suzhou Industrial Park. BEAVER combines biotech with nanotech as a “twin-turbine”strategy in the product design. BEAVER team focuses on magnetic nanoparticle technology and product development, provides customers with professional sample ...