Bovine Sheep Foot Mouth Virus Veterinary Test Kit Antibody Test Strip

Product Specification

Bovine Sheep Foot Mouth Virus Veterinary Test Kit Antibody Test Strip

Bovine and Sheep Foot and Mouth Disease Virus Type O Antibody Test Strip 25 Tests/Kit

1. Principle

This rapid test is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect the antibody against FMD virus in whole blood, serum. After adding sample, the sample move along with the colloidal gold labeling FMD antigen, is there is FMD antibody in the sample, it combines with antigen on T line and show wine-red color; if there is no FMD antibody, then no color reaction.

2. Components

3. Test procedure

1) Collect whole blood, separate serum (centrifuge at 2000-3000 r/min for 5~15min or separate out naturally at 4℃ overnight), or take whole blood with anticoagulants as sample. Take whole blood without anticoagulants as sample is also OK, but must test it immediately.

2) Open the package, take out test card, put it on clean, flat desk.

3) Use dropper to absorb the sample, adding 3 drops sample into “S” mark well slowly drop by drop.

4) Judge the result in 10-20min, it is invalid beyond 20min.

4. Results judgment

1) Positive: control line and test line are both seen wine red, the more the antibody exist, the thicker the color appear;

2) Weak positive: control line and test line are both seen wine red, but the color of test line is very light;

3) Negative: only control line is seen wine red;

4) Invalidation: control line isn’t seen wine red.

5. Consults for diagnosis

1). If not inoculated by the bacterin against porcine FMD virus:

a. test line don’t appear visible wine red show there are no antibodies against porcine FMD virus in tested samples, if the swine are healthy, it is better to vaccinate in time;

b. test line appear visible wine red show the pig was infected with wild virus, should be more observed to validate.

2). if have been inoculated by the bacterin against porcine FMD virus:

a. the color of test line is thicker than the color of the titer 1:32 from comparison card show the high titer of the antibody against porcine FMD virus, which is enough to resist the strong virus;

b. the color of test line is thinner than the color of the titer 1:32 from comparison card show the the titer of the antibody against porcine FMD virus is not enough to resist the strong virus, it is better to perform complementary vaccination.

6. Storage and expiry date

Store at 2 - 30ºC and the shelf life is 24 months.

7. Notice

1) Do not use test card out of date and bad package;

2) Do not use pure water, running water and normal saline as negative control;

3) Store at room temperature (< 30℃) is OK. If store in cool, need return to room temperature firstly, then open package, avoid moisture. Use the test card as soon as possible after open the package.

4) Do not touch the white membrane surface in the middle of test card.

5) To ensure the test quality, take fresh, unpolluted sample. If there is a lot of blood lipids, it will affect the result, so after centrifuge, take serum to test, avoid fat. If the blood or serum is sticky or not enough, use normal saline to dilute at same dilution factor to dilute, then to test.

6) If the serum can not be tested immediately, store at 4℃ for short time (in 48hours), store at below -20℃ for long-tern storage.

7) Whole blood without anticoagulants should be tested immediately; if already exist part of cruor, separate serum to test. The whole blood with anticoagulants should be tested in 24hours, do not frozen. Freezing and thawing causes hemolysis of red blood cells rupture, seriously affect the test results.

8) When dropping sample, keep head of dropper and “S” well at 1cm to ensure adding sample accurately. If too close, can cause small droplet volume caused by drips judgment error or is not allowed to join the sample size, influence the test results.

9) Avoid bubble when adding sample, the sample quantity should be controlled at about 100ul (about 3 drops). Too much sample would not increase sensitivity. In particular, the whole blood of excessive have made erythrocyte sedimentation in "show hole" place, causing membrane chromatography is so deep into the red background, seriously affect the results.

10) This test card is only used for porcine sample, whether it is suitable for other animal, it has not been evaluated at present.

11) The antibody level is only related to darkness of T line, no relation with C line. Only the C line is clean to see, it means the test is valid.

12) Treat the test card and sample at pollutant according to local regulations.

Among livestock, cattle, sheep and pigs are all sensitive, of which cattle are the most susceptible, and people can also get sick. Foot-and-mouth disease is transmitted through direct and indirect contact. The respiratory tract, digestive tract, damaged skin and mucous membrane of susceptible animals can be the portal of infection. Animal products such as meat, milk and fur can carry poison; The secretion, excreta, viscera and even exhaled gas of infected animals, infected cattle and pigs are all contaminated, so transport vehicles and vessels, tools, water sources, pastures, feed, personnel and non-sensitive animals are all important vectors of infection. Susceptibility of animals of different ages varies, with young animals suffering the most serious disease and high mortality.
The disease can occur all the year round, and is easy to spread in spring and autumn. In addition, foot-and-mouth disease of susceptible herds tends to break out periodically.

Symptoms and pathological changes

At the beginning of the disease, the patient had a fever of 41 ℃ and saliva in his mouth. After 1-2 days, blisters of broad bean to walnut size appeared on the inner surface of the lip, gums, tongue and cheek mucosa. Soon, the blisters burst and form a red erosive surface with neat edges. If secondary bacterial infection occurs, the ulcer will occur. When blisters occur in the mouth, the skin between the toes and the hoof crown is red and swollen, and then pale, forming blisters. After the blisters break, the red erosive surface is left, and then scabs. If there is bacterial infection, suppuration will occur, and the hoof will not touch the ground, or even the hoof shell will fall off. The nipple also often has blisters, which leads to rotten spots. When there is secondary infection, it will cause mastitis and stop lactation. Because of sucking milk, the calf's body surface symptoms are not obvious, mainly manifested as hemorrhagic enteritis and myocardial paralysis, with a high mortality rate. Myocardial texture is soft, myocardial degeneration, necrosis, showing pale yellow stripes, or irregular spots, intracardiac and extracardiac membrane bleeding, commonly known as "tiger spot heart".

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.