M1 Aflatoxins ELISA Milk Detection Quality Testing Kit For Milk Powder

Product Specification

Aflatoxins M1 Diagnostic ELISA Kit for Fresh Milk 96 Wells/Kit Sensitivity 0.03

1. Aflatoxins M1 Diagnostic ELISA Kit Principle

This kit is based on the indirect competitive enzyme immunoassay for the detection of aflatoxin M1. Conjugated antigens are pre-coated on microwell strips. Aflatoxin M1 in the sample competes with the conjugated antigen pre-coated on the microwell strip for anti-aflatoxin M1 antibody. After the enzyme conjugate is added, TMB substrate is added for staining. The optical density (OD) value of the sample was negatively correlated with the aflatoxin M1 in the sample. This value is compared to the standard curve and the residual aflatoxin M1 is obtained.

2. Technical Specifications

Sensitivity: 0.03 ppb
Incubator temperature: 25℃
Culture time: 30min~15min
Detection limit:

Cross Reaction Rate:

Recovery rate:

3. Aflatoxins M1 Diagnostic ELISA Kit Components

4. ELISA procedures

4.1Instructions

1. Bring ELISA reagents to room temperature (20 - 25 °C) before use.

2. Put ELISA reagents back to 2-8 ℃immediately after use

3. The ELISA reproducibility in the analysis process is largely depends on the consistency of the washing plate, the correct washing plate operation is the point of determination ELISA program

4. In all process of constant temperature incubation, avoid light exposure, seal the microplate with the cover membrane

4. 2 Operation procedures

1. Bring test kit to the room temperature (20-25 ℃) for at least 30 min, note that each reagent must be shaken evenly before use;

2. Put the required micro-well strips into plate frames. Re-sealed the unused microplate, stored at 2-8 ℃, not frozen.

3. Solution preparation: take the 40ml 20×concentrated washing buffer, dissolve with deionized water at 1:19 (1 part 20×concentrated washing buffer + 19 parts deionized water), or prepare as quantity needed.

4. Numbering: number the micro-wells according to samples and standard solution; each sample and standard solution should be performed in duplicate; record their positions.

5. Add standard/sample: Add 50 µL of the sample or the standard solution into separate duplicate wells, then add enzyme conjugate, 50 µL/well; then antibody working solution, 50 µL/well. Mix gently by shaking the plate manually, seal the microplate with the cover membrane,incubate at25 °C for 30 min in the dark.

6. Wash microplate: Carefully open the cover membrance,pour liquid out of microwell; add 250 µL/well of washing buffer, wash fully for 4-5 times, 15-30 s each time, then take out and flap to dry with absorbent paper.(Use unused spear to pierce bubble after dry)

7. Coloration: add 50 µL of substrate A solution then 50 µL B solution into each well. Mix gently by shaking the plate manually, andincubate at25 °C for 15minin the dark for coloration.

8. Determination: add 50 µL of the stop solution into each well. Mix gently by shaking the plate manually. Set the wavelength of the microplate reader at 450 nm to determine the OD value of every well. (Recommend to read the OD value at the dual-wavelength 450/630 nm within 5 min).

5. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.

Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.