192 Wells/Kit Virus Porcine Test Kit Type A Foot And Mouth Disease Test ISO9001

Product Specification

Competitive ELISA Kit for Detecting Antibody of Foot and Mouth Disease Virus Type A 192 Wells/Kit

1. Usage

Itis usedfor detection of FMD TypeA antibody in serum of pig, bovine and goat, sheep. It is suitable for antibody monitoring and Immune effect assessment.

2. Principle

This kit adopts the competitive ELISA method to detect type A neutralizing antibody, and the ELISA plateis coatedwith FMDV-A VP1 recombinant protein antigen, and the anti-VP1 protein monoclonal antibody is the enzyme marker. Add the serum to be tested to the antigen-coated plate, and then add the enzyme-labeled monoclonal antibody. If there is FMDV-A-specific antibody in the serum to be tested, it will compete with the enzyme marker for the antigen in the coated plate, and the unbound components will be blocked. Wash off, the color is light or no color after adding the substrate. Conversely, the color was darker after adding the substrate. Use a microplate reader to measure the OD450nm value, and determine the experimental results according to the calculation formula.

3. The kit components

4. Materials Required But Not Provided

1) Microplate Reader (single-wave length: 450 nm).
2) Precise micropipette (single-channel 1-100ul,0.5-10ul,multi-channel 30-300ul)
3) Constant temperature box orwater bath box.
4) Oscillator.
5) Disposable tips (10ul, 200ul)
6) Deionized water

5. Sample requirement

Try to use freshly collected serum samples; It cannot be used to detect samples with serious pollution or hemolysis; If the serum sample is turbid, take the supernatant after centrifugation for detection; The test sample can be stored at 2 ~ 8℃for 5 days. If it is stored for a long time, it needs to be transferred to - 20℃or lower temperature.

6. Preparation

1) BringELISA reagents to the room temperature(25±3℃) for at least 30 min to get best results. Microplate should return to room temperature and dry before open package.
2) Washingbuffer preparation: Dilute the10×concentrated washing buffer with deionized water at10 times. (for example: 10ml10×concentrated washing buffer + 90ml deionized water ).

7. Procedure

1) Add sample: add 50 µL of negative control serum (NC) and 50 µL of positive control serum (PC) to the control wells; firstlyadd40 µL of sample diluent to the sample wells, then10 µL of serum to each well;add50μL of Enzyme conjugate to each well, gently shake to mix (do not spill), cover with sealing film, and incubate at 37°C for 30 min.
2) Wash the plate: discard the liquid in the well, add 300μL of dilutedwashing bufferto each well, and wash three times. After the last wash, gently pat the ELISA plate dry on absorbent paper. It is strictly forbidden for the well plate to dry between steps.
3) AddSubstrate: Add100μL of Substrate to each well, mix gently, cover with sealing film, and incubate at 37°C for 10 min in the dark.
4) Termination: Add 50μL of stop solution to each well, shake for 10 seconds, mix well, and read after termination.
5) Reading: Use a microplate reader to measure the optical density value (OD value) at a wavelength of 450nm.

8. Results

1) For the assay to be valid, average OD value of Positive control＜0.4, average OD value of Negative control＞0.6.
2) Calculation method:ODNC Average value =(ODNC1＋ODNC2)/2
3) Sample S/N value calculation formula:S/N＝SampleOD value/ODNC Average value
4) Judgment standard: S/N≤0.5, judged as positive; S/N＞0.5, judged as negative.

9. Precautions and warnings for users

1) Read the Manual carefully before use.
2) Do not use reagents expired, do not mix reagents from different lots.
3) Experiment rubbish should be dealt with high pressure steam sterilization at 121℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes, then discard.
4) MicroWell plate removed from the refrigerated environment should be balanced moisture to dry at room temperature, then can be opened. Put back unused MicroWell plate into dry foil bag and sealed at2-8 ℃. Unused liquid reagent should cover caps, store at 2-8℃in dark with other group components.
5) Should useMicropipettor to add sample and reagents, and often proof its accuracy.
6) When adding washing buffer, should be full but no overflow, avoid appearing free enzyme at mouth of well or cross pollution between wells.
7) Stop solution is corrosive, use large amount of water to wash immediately when touch the skin or clothes.

Specifications:96 wells×2.
Expiry date:12 months.
Storage:Store at 2~8℃, in the dark,no freezing.
Production Date:On outer-packing of the test kit.

For veterinary diagnostic use only

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: Usually by air, we will choose the best shipping method for you, and we can also ship according to the shipping method you require.